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By default, it is assumed that the barcode is located on one of the mates of paired-end read, while cDNA is on the other mate. However, in some scRNA-seq protocols the barcode and cDNA sequences are ...

Contributed by Howard Y. Chang; received November 19, 2024; accepted February 20, 2025; reviewed by Jellert Gaublomme and David A. Quigley The spatial organization of cells within tissues plays a ...